mouse tuj 1  (Neuromics)

Journal: eNeuro

Article Title: The Auditory Brainstem Response Diagnoses Alzheimer-Like Disease in the 5xFAD Mouse Model

doi: 10.1523/ENEURO.0049-25.2025

Figure Lengend Snippet: No differences in peripheral neuronal structures were observed between genotypes. A , Representative view of SGN labeling in 5xFAD and WT mice at 12M. Images show the 16 kHz regions, with labels for DAPI (blue), Calb2 (green), and TUJ1 (red). B , Quantification of Type 1 SGN density (left) and the proportion of Type 1c SGNs (right). C , Representative view of afferent and efferent terminal labeling in cochlear whole mounts from 5xFAD and WT mice at 12M, with labels for CtBP2 (green) and VAT (magenta). Tonotopic cochlear regions for 8 kHz (left column) and 16 kHz (right column) are shown. D , Quantification of CtBP2 puncta number per IHC (left) and mean VAT terminal volume per IHC (right). E , Representative view of efferent terminal labeling (VAT in white color) in cochlear whole mounts from 5xFAD and WT mice at 12M. The 8 (top row), 16 (middle row), and 32 (bottom row) kHz regions are shown. F , Quantification of VAT terminal volume (µm 3 ) per OHC. Asterisks denote the significance of differences between WT and 5xFAD mice: no significance (ns), p ≥ 0.05; * p < 0.05; ** p < 0.01; and *** p < 0.001, Student's t test or Wilcoxon test. Scale bar, 10 μm for A , C , and E .

Article Snippet: The following primary antibodies were used in this analysis: mouse TUJ1 (anti-beta Tubulin 3, 1:100, R&D Systems) and mouse anti- calbindin 2 (Calb2, 1:100, Millipore Sigma).

Techniques: Labeling

Journal: Neural Regeneration Research

Article Title: Maintaining moderate levels of hypochlorous acid promotes neural stem cell proliferation and differentiation in the recovery phase of stroke

doi: 10.4103/1673-5374.392889

Figure Lengend Snippet: Moderate hypochlorous acid promotes neural stem cell differentiation into astrocytes and neurons under O/R conditions. (A, B) Effect and statistical analysis of low concentration hypochlorous acid on the expression of GFAP, Tuj-1, and CNP detected by western blotting in C17.2 cells. Data were normalized to the Control (Con) group. (C, D) Effect and statistical analysis of low concentration hypochlorous acid on expression of GFAP, Tuj-1, and CNP detected by western blotting in O/R-challenged C17.2 cells. Data were normalized to the Con group. (E–H) Representative images and statistical analysis of low concentration hypochlorous acid on immunopositivity of GFAP (PE, red), Tuj-1 (FITC, green), and CNP (FITC, green) in O/R-challenged primary NSCs. 5 μM hypochlorous acid increased the ratio of GFAP + to DAPI + cells, but reduced the ratio of CNP + to DAPI + cells. However, 100 μM hypochlorous acid increased the ratio of Tuj-1 + to DAPI + cells and GFAP + to DAPI + cells, but reduced the ratio of CNP + to DAPI + cells. (I, J) Representative images and statistical analysis of low concentration hypochlorous acid on immunopositivity of Tuj-1 (FITC, green) in the SVZ of MCAO rats. Low-concentration hypochlorous acid increased Tuj-1 immunopositivity. Data were normalized to the MCAO group. Scale bar: 50 μm. Data are expressed as mean ± SD ( n = 5). * P < 0.05, ** P < 0.01, *** P < 0.001 (B, F–H: one-way analysis of variance followed by Dunnett’s post hoc test, D, J: one-way analysis of variance followed by Tukey’s post hoc test). CNP: 2′,3′-Cyclic nucleotide 3′-phosphodiesterase; DAPI: 4′,6-diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; MCAO: middle cerebral artery occlusion; NSC: neural stem cell; O/R: oxygen-glucose deprivation/reoxygenation; SVZ: subventricular zone; Tuj-1: beta-III tubulin.

Article Snippet: Primary antibodies were incubated overnight at 4°C: mouse anti-rat Nestin (1:200, Cat# NBP1-92717, Novus Biologicals, Littleton, CO, USA, RRID: AB_11020601), rabbit anti-rat Sox2 (1:200, Cat# AF2018, R&D Systems, Minneapolis, MN, USA, RRID: AB_355110), rabbit ionized calcium-binding adapter molecule 1 (Iba-1, 1:100, Cat# A19776, Abclone, Wuhan, China), phycoerythrin mouse anti-GFAP (1:200, Cat# 16825-1-AP, BD Biosciences, Franklin Lake, NJ, USA, RRID: AB_1645412), mouse anti-2,3-cyclic nucleotide 3-phosphodiesterase (CNP, 1:100, Cat# 66729-1-IG, Abcam, RRID: AB_2082593), and mouse beta-III tubulin (Tuj-1) (FITC) (1:200, Cat# 66375-1-IG, Novus Biologicals, RRID: AB_535885).

Techniques: Cell Differentiation, Concentration Assay, Expressing, Western Blot, Control